Journal: Nutrients
Article Title: Hepatic-Metabolic Activity of α-Lipoic Acid—Its Influence on Sphingolipid Metabolism and PI3K/Akt/mTOR Pathway in a Rat Model of Metabolic Dysfunction-Associated Steatotic Liver Disease
doi: 10.3390/nu16101501
Figure Lengend Snippet: The hepatic content of phosphorylated proteins from insulin transduction pathway, i.e., insulin receptor substrate 1 (pIRS1(Ser636/Ser639), ( A )), phosphate and tensin homolog (pPTEN(Ser380), ( B )), protein kinase B (pAkt(Ser473), ( C )), glycogen synthase kinase 3α/β (pGSK3α/β(Ser21/Ser9), ( D )), Bcl-2-associated agonist of cell death (pBAD(Ser136), ( E )), mechanistic target of rapamycin (pmTOR(Ser2448), ( F )), P70 ribosomal protein S6 kinase (pP70 S6 kinase(Thr389), ( G )) and S6 ribosomal protein (pS6RP(Ser235/Ser236), ( H )) in rats subjected to a standard diet (Control) or a high-fat diet (HFD) after α-lipoic acid (α-LA) administration for eight weeks. Magnetic bead-related immunoassay-measured phosphorylated proteins’ fluorescent intensity (FI). The results are presented as mean ± standard deviation (SD) and established on ten independent determinations in each experimental group. * p < 0.05—significant differences vs. Control group. # p < 0.05—significant differences vs. HFD group.
Article Snippet: The hepatic level of phosphorylated proteins from the insulin signaling pathway, i.e., insulin receptor substrate 1 (pIRS1(Ser636/Ser639)), phosphate and tensin homolog (pPTEN(Ser380)), protein kinase B (pAkt(Ser473)), glycogen synthase kinase 3α/β (pGSK3α/β(Ser21/Ser9)), Bcl-2-associated agonist of cell death (pBAD(Ser136)), mechanistic target of rapamycin (pmTOR(Ser2448)), P70 ribosomal protein S6 kinase (pP70 S6 kinase(Thr389)) and S6 ribosomal protein (pS6RP(Ser235/Ser236)) was assessed using a Bio-Plex Pro Cell Signaling Assays (Bio-Rad, Hercules, CA, USA).
Techniques: Transduction, Control, Standard Deviation